Journal: STAR Protocols

Article Title: Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo

doi: 10.1016/j.xpro.2022.101940

Figure Lengend Snippet:

Article Snippet: RPMI Medium 1640 basic (1×) , Gibco , C22400500BT.

Techniques: Recombinant, DNA Extraction, Lysis, Modification, Cell Culture, Software, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo

doi: 10.1016/j.xpro.2022.101940

Figure Lengend Snippet: Culture medium (complete 1640 medium)

Article Snippet: RPMI Medium 1640 basic (1×) , Gibco , C22400500BT.

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps

doi: 10.3389/fimmu.2022.1046426

Figure Lengend Snippet: Multiplex immunofluorescence staining of nasal tissues for MBP (red), Siglec8 (orange), CRTH2 (green), and DAPI (blue) were performed. Representative fluorescence images of rNP group, Non-rNP group, and control group. White Arrows indicated eosinophils that expressed the corresponding markers. C, control group.

Article Snippet: At room temperature, we incubated primary antibodies for major basic protein (MBP) (1:150; Boster, China), Siglec8 (1:30; Biolegend, USA), CRTH2 (1:150; Abcam, China), CD69 (1:150; Santa Cruz Biotechnology, China) for 2 hours.

Techniques: Multiplex Assay, Immunofluorescence, Staining, Fluorescence, Control

Journal: Frontiers in Immunology

Article Title: Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps

doi: 10.3389/fimmu.2022.1046426

Figure Lengend Snippet: Representative tissue images stained using multiplex immunofluorescence staining for CRTH2 (red), Siglec8 (orange), MBP (yellow), CD69 (green), and DAPI (blue) in rNP group, Non-rNP group, and control group. White Arrows indicated eosinophils that expressed the corresponding markers. C, control group.

Article Snippet: At room temperature, we incubated primary antibodies for major basic protein (MBP) (1:150; Boster, China), Siglec8 (1:30; Biolegend, USA), CRTH2 (1:150; Abcam, China), CD69 (1:150; Santa Cruz Biotechnology, China) for 2 hours.

Techniques: Staining, Multiplex Assay, Immunofluorescence, Control